January 2021 Case Cedars-Sinai Skip to content Close Select your preferred language English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog English English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog Translation is unavailable for Internet Explorer Cedars-Sinai Home 1-800-CEDARS-1 1-800-CEDARS-1 Close Find a Doctor Locations Programs & Services Health Library Patient & Visitors Community My CS-Link Education clear Go Close Academics Academics Faculty Development Community Engagement Calendar Research Research Areas Research Labs Departments & Institutes Find Clinical Trials Research Cores Research Administration Basic Science Research Clinical & Translational Research Center (CTRC) Technology & Innovations News & Breakthroughs Education Graduate Medical Education Continuing Medical Education Graduate School of Biomedical Sciences Professional Training Programs Medical Students Campus Life Office of the Dean Simulation Center Medical Library Program in the History of Medicine About Us All Education Programs Departments & Institutes Faculty Directory Anatomic and Clinical Pathology Residency Back to Anatomic and Clinical Pathology Residency Application Information Explore the Residency Training Curriculum Autopsy Pathology Rotation Bone and Soft Tissue Head and Neck Pathology Rotation Breast Pathology Rotation Cardiovascular Pathology Rotation Clinical Chemistry Rotation Coagulation Rotation Cytopathology Rotation Dermatopathology Rotation Forensic Pathology Rotation Frozen Section Rotation Gastrointestinal and Liver Pathology Genitourinary Pathology Rotation Genomic Pathology Rotation Gynecologic Pathology Rotation Hematopathology Rotation Laboratory Management Rotation Microbiology Rotation Neuropathology Rotation Pulmonary and Mediastinal Pathology Rotation Renal Pathology Rotation Transfusion Medicine Rotation Surgical Pathology Pathology Physician Scientist Training Program Residents Graduates Case of the Month Archive Publications Leadership Frequently Asked Questions January 2021 Case Authors Kevin Song, MD (Fellow) and Serhan Alkan, MD (Faculty) Hematopathology Clinical History Middle aged male patient, otherwise healthy with only minor health issues, presented with progressive cervical lymphadenopathy. Imaging showed numerous enlarged lymph node throughout his neck, measuring up to 4.5 cm. He did not have any systemic B-symptoms. Further examination showed mild splenomegaly and mildly enlarged lymph nodes in the chest, abdomen, and pelvis. He had a fine needle aspiration performed which was inconclusive (atypical lymphoid hyperplasia). His labs at initial consultation showed WBC of 16.24 x 10³/µL, Hgb 12.5 g/dL, and platelets 197 x 10⁶/µL. No differential was available, however the previous CBC demonstrated increased lymphocytes. Additionally, his labs showed a mild increase in AST and ALT, reaching up to 172 and 288 soon after his initial visit. A lymph node biopsy is performed, followed by a liver biopsy and a bone marrow biopsy. Lymph node biopsy H & E CD20+ B-cells CD4+ T-cells CD30 PD1 TIA1 ALK A diffuse proliferation of small-medium cells is seen with loss of nodal architecture. They are CD4+ T-cells with loss of CD5 and CD3 (partial). CD30 (variable), CD43, granzyme, TIA1, and ALK are positive; CD8, PD1, CXCL13, CD10, BCL6 and TCL1 are negative. The immunophenotype and features are consistent with small cell variant of anaplastic large cell lymphoma. Discussion Anaplastic Large Cell Lymphoma Anaplastic large cell lymphoma (ALCL) is a T-cell lymphoma with systemic ALK positive and negative types among others. They have variable morphology, including several different histological patterns described in ALK+ ALCL. ALK positive cases typically affect younger patients and are associated with a better prognosis while ALK negative lymphomas affect older individuals with worse outcomes. The most common translocation for ALK+ cases is t(2;5) in approximately 80% of cases, however other fusion partners for ALK also exist on a number of other chromosomes. DUSP22 (6p25) and TP63 rearrangements may occur in ALK negative cases. Prognostically, positivity p63 conveys a worse prognosis while the presence of DUSP22 rearrangement is associated with a better outcome. In the lymph node, the principal T-cell lymphoma differential diagnosis includes peripheral T-cell lymphoma NOS (PTCL-NOS) and angioimmunoblastic T-cell lymphoma (AITL). A key feature of ALCL's are pleomorphic, horseshoe-shaped "hallmark" cells which may be variable in size. The tumor cells are strongly CD30 positive (with large cells typically stronger than small cells), unlike the other entities previously mentioned. Additionally, ALCL expresses cytotoxic markers such as TIA-1 (unlike PTCL-NOS) but do not express follicular helper cell markers such as PD1 or have expanded/disrupted follicular dendritic cell networks (unlike AITL). ALK+ ALCL can demonstrate several different histological patterns. The "classic" pattern is the most common (approximately 60% of cases) in which large pleomorphic cells predominate. Other less common patterns include lymphohistiocytic (10%), Hodgkin's like (3%), small cell (5-10%) or composite patterns. The case presented above is an example of small cell pattern. There is a diffuse proliferation of mostly small to medium cells, with some showing a moderate amount of pale cytoplasm. In this case, most cells are CD4 positive and a subset (medium and few large cells) strongly express CD30 and ALK. It is important to note that hallmark cells may be absent in the small cell variant of ALCL. Therefore, immunohistochemistry for ALK will be crucial as diagnosis will be challenging without ALK staining. The small cells may be weak or negative for CD30 (as we can see in a subset in the CD30 photomicrograph above). Together with the negative PD1 and positive cytotoxic markers, the immunophenotype in this case is consistent with ALK+ ALCL. Another potential pitfall seen in subsequent cases from this patient is subtle sinusoidal involvement (illustrated below). Lymph node sinusoidal disease is recognized in ALCL and may be misdiagnosed as a carcinoma or other neoplasm. In this patient, there was a subtle population of small lymphocytes within the sinusoids of the liver (see images below). These consisted of a mixture of CD4 and CD8 T-cells, a subset of which expressed cytotoxic markers and CD30. A subset also expressed ALK, which is consistent with involvement by ALCL. A similar pattern of disease was also seen in the bone marrow, where focal small ALK+ T-cells were present which were otherwise not noticeable on routine H&E slides. Liver biopsy H&E high power ALK References Swerdlow, SH., Campo, E et al. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues (Revised 4th Edition). IARC: Lyon 2017 Montes-Mojarro, IA., Steinhilber, J et. al. The Pathological Spectrum of Systemic Anaplastic Large Cell Lymphoma (ALCL). Cancers (Basel) 2018 Apr; 10(4): 107 Hapgood, G., Savage, KJ. The biology and management of systemic anaplastic large cell lymphoma. Blood 2015; 126 (1): 17–25 Pletneva, MA., Smith, LB. Anaplastic Large Cell Lymphoma: Features Presenting Diagnostic Challenges. Arch Pathol Lab Med 1 October 2014; 138 (10): 1290–1294 Please ensure Javascript is enabled for purposes of website accessibility