Research Areas Jorth Lab Cedars Sinai

Research Areas Jorth Lab Cedars Sinai

Research Areas - Jorth Lab Cedars-Sinai Skip to content Close Select your preferred language English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog English English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog Translation is unavailable for Internet Explorer Cedars-Sinai Home 1-800-CEDARS-1 1-800-CEDARS-1 Close Find a Doctor Locations Programs & Services Health Library Patient & Visitors Community My CS-Link RESEARCH clear Go Close Navigation Links Academics Faculty Development Community Engagement Calendar Research Research Areas Research Labs Departments & Institutes Find Clinical Trials Research Cores Research Administration Basic Science Research Clinical & Translational Research Center (CTRC) Technology & Innovations News & Breakthroughs Education Graduate Medical Education Continuing Medical Education Graduate School of Biomedical Sciences Professional Training Programs Medical Students Campus Life Office of the Dean Simulation Center Medical Library Program in the History of Medicine About Us All Education Programs Departments & Institutes Faculty Directory Jorth Lab Back to Jorth Lab Personal Statement Research Areas Lab Members Publications Research Areas Evolution During Chronic Cystic Fibrosis Infection and Antibiotic Resistance The Jorth Laboratory is interested in how microbes evolve during infection to escape the immune response and resist antibiotic treatment. Individuals with cystic fibrosis (CF) suffer from lifelong chronic bacterial lung infections, primarily caused by Pseudomonas aeruginosa (P. aeruginosa). The Jorth Lab uses genomic approaches to: Identify mutations that arise in bacteria collected from CF lung infections Discover mutations that arise in laboratory-evolved antibiotic resistant bacteria P. aeruginosa biofilm aggregates grown in an agar block biofilm assay. Bacteria (green) were stained with HCR nucleic acid probes and imaged by confocal microscopy after 16 hours growth. Biofilms promote bacterial tolerance to antibiotic treatment and play an important role during chronic CF infections. Biofilm aggregates growing near oxygen are larger at the top of the agar block than aggregates growing with less oxygen near the bottom of the agar block. P. aeruginosa biofilm aggregates grown in an agar block biofilm assay. Bacteria (green) were stained with HCR nucleic acid probes and imaged by confocal microscopy after 16 hours growth. Aggregate surfaces were reconstructed from HCR nucleic acid staining. Virulence and Host-Pathogen Interactions The Jorth Lab recently discovered a set of P. aeruginosa virulence-enhancing resistance mutations (VERMs) that confer aztreonam antibiotic resistance and increase P. aeruginosa virulence, even in the absence of antibiotics. Lab members are working to understand the molecular mechanisms by which these VERMs simultaneously make this bacterium more resistant to treatment and lethal to the host. Bacterial biofilm in CF lung tissue. Image captured by confocal microscopy after tissue clearing, immunofluorescence staining for P. aeruginosa (orange), and DAPI staining of host nuclei (blue). Bacteria inside human cells CF sputum. Image captured by confocal microscopy after tissue clearing, hybridization chain reaction (HCR) nucleic acid staining for P. aeruginosa (orange), DAPI staining of host nuclei (blue), lectin staining of host mucin (white). Microbiome The human body is essentially a culture vessel for countless microbes that colonize our bodies. In addition to causing disease, these organisms play essential roles in immune system development and help protect us from infection by invading pathogens. The Jorth Laboratory is interested in how the microbiome changes both compositionally and functionally during different disease states and treatments. We have experience studying the lung and sputum microbiota in people with CF, as well as the periodontal microbiota in people with periodontal disease. Contact the Jorth Lab 127 S. San Vicente Blvd. Advanced Health Sciences Pavilion, Room 9600 Los Angeles, CA 90048 424-315-4590 Send a Message Please ensure Javascript is enabled for purposes of website accessibility
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