Publications Cedars-Sinai Skip to content Close Select your preferred language English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog English English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog Translation is unavailable for Internet Explorer Cedars-Sinai Home 1-800-CEDARS-1 1-800-CEDARS-1 Close Find a Doctor Locations Programs & Services Health Library Patient & Visitors Community My CS-Link RESEARCH clear Go Close Navigation Links Academics Faculty Development Community Engagement Calendar Research Research Areas Research Labs Departments & Institutes Find Clinical Trials Research Cores Research Administration Basic Science Research Clinical & Translational Research Center (CTRC) Technology & Innovations News & Breakthroughs Education Graduate Medical Education Continuing Medical Education Graduate School of Biomedical Sciences Professional Training Programs Medical Students Campus Life Office of the Dean Simulation Center Medical Library Program in the History of Medicine About Us All Education Programs Departments & Institutes Faculty Directory Imaging Mass Cytometry Core Back to Imaging Mass Cytometry Core Meet the Team Protocols Instrumentation Vendor List Publications FAQs Publications Technology and Applications Doan H, Chinn GM, Jahan-Tigh RR. Flow Cytometry II: Mass and Imaging Cytometry. J Invest Dermatol. 2015 Sep;135(9):1-4. doi: 10.1038/jid.2015.263. Erratum in: J Invest Dermatol. 2015 Dec;135(12):3204. PMID: 26269409. Gerdtsson E, Pore M, Thiele JA, Gerdtsson AS, Malihi PD, Nevarez R, Kolatkar A, Velasco CR, Wix S, Singh M, Carlsson A, Zurita AJ, Logothetis C, Merchant AA, Hicks J, Kuhn P. Multiplex protein detection on circulating tumor cells from liquid biopsies using imaging mass cytometry. Converg Sci Phys Oncol. 2018 Mar;4(1):015002. doi: 10.1088/2057-1739/aaa013. Epub 2018 Jan 16. PMID: 30906572; PMCID: PMC6430142. Chang Q, Ornatsky OI, Siddiqui I, Loboda A, Baranov VI, Hedley DW. Imaging Mass Cytometry. Imaging Cytometry A. 2017 Feb;91(2):160-169. doi: 10.1002/cyto.a.23053. Epub 2017 Feb 3. PMID: 28160444. Ijsselsteijn ME, van der Breggen R, Farina Sarasqueta A, Koning F, de Miranda NFCC. A 40-Marker Panel for High Dimensional Characterization of Cancer Immune Microenvironments by Imaging Mass Cytometry. Front Immunol. 2019 Oct 29;10:2534. doi: 10.3389/fimmu.2019.02534. PMID: 31736961; PMCID: PMC6830340. Baharlou H, Canete NP, Cunningham AL, Harman AN, Patrick E. Mass Cytometry Imaging for the Study of Human Diseases-Applications and Data Analysis Strategies. Front Immunol. 2019 Nov 14;10:2657. doi: 10.3389/fimmu.2019.02657. PMID: 31798587; PMCID: PMC6868098. Hartmann FJ, Bendall SC. Immune monitoring using mass cytometry and related high-dimensional imaging approaches. Nat Rev Rheumatol. 2020 Feb;16(2):87-99. doi: 10.1038/s41584-019-0338-z. Epub 2019 Dec 31. PMID: 31892734; PMCID: PMC7232872. Han G, Spitzer MH, Bendall SC, Fantl WJ, Nolan GP. Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry. Nat Protoc. 2018 Oct;13(10):2121-2148. doi: 10.1038/s41596-018-0016-7. PMID: 30258176; PMCID: PMC7075473. Gadalla R, Noamani B, MacLeod BL, Dickson RJ, Guo M, Xu W, Lukhele S, Elsaesser HJ, Razak ARA, Hirano N, McGaha TL, Wang B, Butler M, Guidos CJ, Ohashi PS, Siu LL, Brooks DG. Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials. Front Oncol. 2019 May 17;9:415. doi: 10.3389/fonc.2019.00415. PMID: 31165047; PMCID: PMC6534060. Hartmann FJ, Babdor J, Gherardini PF, Amir ED, Jones K, Sahaf B, Marquez DM, Krutzik P, O'Donnell E, Sigal N, Maecker HT, Meyer E, Spitzer MH, Bendall SC. Comprehensive Immune Monitoring of Clinical Trials to Advance Human Immunotherapy. Cell Rep. 2019 Jul 16;28(3):819-831.e4. doi: 10.1016/j.celrep.2019.06.049. PMID: 31315057; PMCID: PMC6656694. Pomerantz AK, Sari-Sarraf F, Grove KJ, Pedro L, Rudewicz PJ, Fathman JW, Krucker T. Enabling Drug Discovery and Development Through Single-Cell Imaging. Expert Opin Drug Discov. 2019 Feb;14(2):115-125. doi: 10.1080/17460441.2019.1559147. Epub 2018 Dec 24. PMID: 30583700. Bouzekri A, Esch A, Ornatsky O. Multidimensional Profiling of Drug-Treated Cells by Imaging Mass Cytometry. FEBS Open Bio. 2019 Sep;9(9):1652-1669. doi: 10.1002/2211-5463.12692. Epub 2019 Jul 21. PMID: 31250984; PMCID: PMC6722888. Methodology and Experimental Design Rybakowska P, Alarcón-Riquelme ME, Marañón C. Key steps and methods in the experimental design and data analysis of highly multi-parametric flow and mass cytometry. Comput Struct Biotechnol J. 2020 Mar 31;18:874-886. doi: 10.1016/j.csbj.2020.03.024. PMID: 32322369; PMCID: PMC7163213. Gullaksen SE, Bader L, Hellesøy M, Sulen A, Fagerholt OHE, Engen CB, Skavland J, Gjertsen BT, Gavasso S. Titrating Complex Mass Cytometry Panels. Cytometry A. 2019 Jul;95(7):792-796. doi: 10.1002/cyto.a.23751. Epub 2019 Apr 9. PMID: 30964237; PMCID: PMC6766997. Kleinsteuber K, Corleis B, Rashidi N, Nchinda N, Lisanti A, Cho JL, Medoff BD, Kwon D, Walker BD. Standardization and Quality Control for High-Dimensional Mass Cytometry Studies of Human Samples. Cytometry A. 2016 Oct;89(10):903-913. doi: 10.1002/cyto.a.22935. Epub 2016 Aug 30. PMID: 27575385; PMCID: PMC5495108. Han G, Spitzer MH, Bendall SC, Fantl WJ, Nolan GP. Method for tagging antibodies with metals for mass cytometry experiments. Nat Protoc. 2018 Oct;13(10):2121-2148. doi: 10.1038/s41596-018-0016-7. PMID: 30258176; PMCID: PMC7075473. Chevrier S, Crowell HL, Zanotelli VRT, Engler S, Robinson MD, Bodenmiller B. Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry. Cell Syst. 2018 May 23;6(5):612-620.e5. doi: 10.1016/j.cels.2018.02.010. Epub 2018 Mar 28. PMID: 29605184; PMCID: PMC5981006. Catena R, Montuenga LM, Bodenmiller B. Ruthenium Counterstaining for Imaging Mass Cytometry. J Pathol. 2018 Apr;244(4):479-484. doi: 10.1002/path.5049. Epub 2018 Mar 6. PMID: 29405336. Chang Q, Ornatsky O, Hedley D. Staining of Frozen and Formalin-Fixed, Paraffin-Embedded Tissues with Metal-Labeled Antibodies for Imaging Mass Cytometry Analysis. Curr Protoc Cytom. 2017 Oct 2;82:12.47.1-12.47.8. doi: 10.1002/cpcy.29. PMID: 28967989. Decalf J, Albert ML, Ziai J. New Tools for Pathology: A User's Review of a Highly Multiplexed Method for in Situ Analysis of Protein and RNA Expression in Tissue. J Pathol. 2019 Apr;247(5):650-661. doi: 10.1002/path.5223. Epub 2019 Feb 20. PMID: 30570141. Singh N, Avigan ZM, Kliegel JA, Shuch BM, Montgomery RR, Moeckel GW, Cantley LG. Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry. JCI Insight. 2019 Jun 20;4(12):e129477. doi:10.1172/jci.insight.129477. PMID: 31217358; PMCID: PMC6629112. Suspension Cell Mass Cytometry Helmink BA, Reddy SM, Gao J, Zhang S, Basar R, Thakur R, Yizhak K, Sade-Feldman M, Blando J, Han G, Gopalakrishnan V, Xi Y, Zhao H, Amaria RN, Tawbi HA, Cogdill AP, Liu W, LeBleu VS, Kugeratski FG, Patel S, Davies MA, Hwu P, Lee JE, Gershenwald JE, Lucci A, Arora R, Woodman S, Keung EZ, Gaudreau PO, Reuben A, Spencer CN, Burton EM, Haydu LE, Lazar AJ, Zapassodi R, Hudgens CW, Ledesma DA, Ong S, Bailey M, Warren S, Rao D, Krijgsman O, Rozeman EA, Peeper D, Blank CU, Schumacher TN, Butterfield LH, Zelazowska MA, McBride KM, Kalluri R, Allison J, Petitprez F, Fridman WH, Sautès-Fridman C, Hacohen N, Rezvani K, Sharma P, Tetzlaff MT, Wang L, Wargo JA.B Cells and Tertiary Lymphoid Structures Promote Immunotherapy Response. Nature. 2020 Jan;577(7791):549-555. doi: 10.1038/s41586-019-1922-8. Epub 2020 Jan 15. PMID: 31942075. Spitzer MH, Carmi Y, Reticker-Flynn NE, Kwek SS, Madhireddy D, Martins MM, Gherardini PF, Prestwood TR, Chabon J, Bendall SC, Fong L, Nolan GP, Engleman EG. Systemic Immunity is Required for Effective Cancer Immunotherapy. Cell. 2017 Jan 26;168(3):487-502.e15. doi: 10.1016/j.cell.2016.12.022. Epub 2017 Jan 19. PMID: 28111070; PMCID: PMC5312823. Gubin MM, Esaulova E, Ward JP, Malkova ON, Runci D, Wong P, Noguchi T, Arthur CD, Meng W, Alspach E, Medrano RFV, Fronick C, Fehlings M, Newell EW, Fulton RS, Sheehan KCF, Oh ST, Schreiber RD, Artyomov MN. High Dimensional Analysis Delineates Myeloid and Lymphoid Compartment Remodeling During Successful Immune Checkpoint Cancer Therapy. Cell. 2018 Nov 1;175(4):1014-1030.e19. doi: 10.1016/j.cell.2018.09.030. Epub 2018 Oct 18. Erratum in: Cell. 2018 Nov 15;175(5):1443. PMID: 30343900; PMCID: PMC6501221. Kay AW, Strauss-Albee DM, Blish CA. Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells. Methods Mol Biol. 2016;1441:13-26. doi: 10.1007/978-1-4939-3684-7_2. PMID: 27177653; PMCID: PMC5304457. Leng Z, Zhu R, Hou W, Feng Y, Yang Y, Han Q, Shan G, Meng F, Du D, Wang S, Fan J, Wang W, Deng L, Shi H, Li H, Hu Z, Zhang F, Gao J, Liu H, Li X, Zhao Y, Yin K, He X, Gao Z, Wang Y, Yang B, Jin R, Stambler I, Lim LW, Su H, Moskalev A, Cano A, Chakrabarti S, Min KJ, Ellison-Hughes G, Caruso C, Jin K, Zhao RC. Transplantation of ACE2-Mesenchymal Stem Cells Improves the Outcome of Patients with COVID-19 Pneumonia. Aging Dis. 2020 Mar 9;11(2):216-228. doi: 10.14336/AD.2020.0228. PMID: 32257537; PMCID: PMC7069465. Bengsch B, Ohtani T, Herati RS, Bovenschen N, Chang KM, Wherry EJ. Deep immune profiling by mass cytometry links human T and NK cell differentiation and cytotoxic molecule expression patterns. J Immunol Methods. 2018 Feb;453:3-10. doi: 10.1016/j.jim.2017.03.009. Epub 2017 Mar 19. PMID: 28322863; PMCID: PMC5605401. IMC-Related Publications Schulz D, Zanotelli VRT, Fischer JR, Schapiro D, Engler S, Lun XK, Jackson HW, Bodenmiller B. Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry. Cell Syst. 2018 Jan 24;6(1):25-36.e5. doi: 10.1016/j.cels.2017.12.001. Epub 2017 Dec 27. Erratum in: Cell Syst. 2018 Apr 25;6(4):531. PMID: 29289569; PMCID: PMC5791659. Jackson HW, Fischer JR, Zanotelli VRT, Ali HR, Mechera R, Soysal SD, Moch H, Muenst S, Varga Z, Weber WP, Bodenmiller B. The single-cell pathology landscape of breast cancer. Nature. 2020 Feb;578(7796):615-620. doi: 10.1038/s41586-019-1876-x. Epub 2020 Jan 20. PMID: 31959985. Damond N, Engler S, Zanotelli VRT, Schapiro D, Wasserfall CH, Kusmartseva I, Nick HS, Thorel F, Herrera PL, Atkinson MA, Bodenmiller B. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry. Cell Metab. 2019 Mar 5;29(3):755-768.e5. doi: 10.1016/j.cmet.2018.11.014. Epub 2019 Jan 31. PMID: 30713109; PMCID: PMC6821395. Wang YJ, Traum D, Schug J, Gao L, Liu C; HPAP Consortium, Atkinson MA, Powers AC, Feldman MD, Naji A, Chang KM, Kaestner KH. Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes. Cell Metab. 2019 Mar 5;29(3):769-783.e4. doi: 10.1016/j.cmet.2019.01.003. Epub 2019 Jan 31. PMID: 30713110; PMCID: PMC6436557. Krams SM, Schaffert S, Lau AH, Martinez OM. Applying Mass Cytometry to the Analysis of Lymphoid Populations in Transplantation. Am J Transplant. 2017 Aug;17(8):1992-1999. doi: 10.1111/ajt.14145. Epub 2016 Dec 23. PMID: 27888565; PMCID: PMC5526773. Theil D, Smith P, Huck C, Gilbart Y, Kakarieka A, Leppert D, Rauld C, Schmid C, Baumgartner R, Stuber N, Cordoba F, Dubost V, Darribat K, Jivkov M, Frieauff W, Kneuer R, Stoeckli M, Reinker S, Mansfield K, Carballido JM, Couttet P, Weckbecker G. Imaging Mass Cytometry and Single-Cell Genomics Reveal Differential Depletion and Repletion of B-Cell Populations Following Ofatumumab Treatment in Cynomolgus Monkeys. Front Immunol. 2019 Jun 20;10:1340. doi: 10.3389/fimmu.2019.01340. PMID: 31281311; PMCID: PMC6596277. Analysis Kimball AK, Oko LM, Bullock BL, Nemenoff RA, van Dyk LF, Clambey ET. A Beginner's Guide to Analyzing and Visualizing Mass Cytometry Data. J Immunol. 2018 Jan 1;200(1):3-22. doi: 10.4049/jimmunol.1701494. PMID: 29255085; PMCID: PMC5765874. Schapiro D, Jackson HW, Raghuraman S, Fischer JR, Zanotelli VRT, Schulz D,Giesen C, Catena R, Varga Z, Bodenmiller B. histoCAT: analysis of cellphenotypes and interactions in multiplex image cytometry data. Nat Methods. 2017 Sep;14(9):873-876. doi: 10.1038/nmeth.4391. Epub 2017 Aug 7. PMID: 28783155; PMCID: PMC5617107. Somarakis A, Van Unen V, Koning F, Lelieveldt BPF, Hollt T. ImaCytE: Visual Exploration of Cellular Microenvironments for Imaging Mass Cytometry Data. IEEE Trans Vis Comput Graph. 2019 Jul 29. doi: 10.1109/TVCG.2019.2931299. Epub ahead of print. PMID: 31369380. Palit S, Heuser C, de Almeida GP, Theis FJ, Zielinski CE. Meeting the Challenges of High-Dimensional Single-Cell Data Analysis in Immunology. Front Immunol. 2019 Jul 3;10:1515. doi: 10.3389/fimmu.2019.01515. PMID: 31354705; PMCID: PMC6634245. Liu P, Liu S, Fang Y, Xue X, Zou J, Tseng G, Konnikova L. Recent Advances in Computer-Assisted Algorithms for Cell Subtype Identification of Cytometry Data. Front Cell Dev Biol. 2020 Apr 28;8:234. doi: 10.3389/fcell.2020.00234. PMID: 32411698; PMCID: PMC7198724. Have Questions or Need Help Contact us if you have questions or would like to learn more about the Imaging Mass Cytometry Core at Cedars-Sinai. Imaging Mass Cytometry Core 127 S San Vicente Blvd. AHSP A8700 Los Angeles, CA 90048 310-423-1078 Send a Message Please ensure Javascript is enabled for purposes of website accessibility