FAQs Cedars-Sinai Skip to content Close Select your preferred language English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog English English عربى 简体中文 繁體中文 فارسي עִברִית 日本語 한국어 Русский Español Tagalog Translation is unavailable for Internet Explorer Cedars-Sinai Home 1-800-CEDARS-1 1-800-CEDARS-1 Close Find a Doctor Locations Programs & Services Health Library Patient & Visitors Community My CS-Link RESEARCH clear Go Close Navigation Links Academics Faculty Development Community Engagement Calendar Research Research Areas Research Labs Departments & Institutes Find Clinical Trials Research Cores Research Administration Basic Science Research Clinical & Translational Research Center (CTRC) Technology & Innovations News & Breakthroughs Education Graduate Medical Education Continuing Medical Education Graduate School of Biomedical Sciences Professional Training Programs Medical Students Campus Life Office of the Dean Simulation Center Medical Library Program in the History of Medicine About Us All Education Programs Departments & Institutes Faculty Directory Rodent Genetics Core Back to Rodent Genetics Core New User Information Instrumentation Services and Prices Scheduling Meet the Team FAQs FAQs and Resources How long does it take to produce transgenic founders? You will receive weaned founders six weeks after DNA microinjection. How long does it take to rederive a mouse line? You will receive weaned progeny six to eight weeks after in vitro fertilization. This is shorter than a typical quarantine process and the resulting mice are pathogen-free. I would like to have a cohort of 50 age-matched mice for my study. We should be able to produce as many as 100 pups within a week of range by in vitro fertilization. Our male breeders are not breeding and they are getting fat and old. Also, we keep losing newborn mice by cannibalism of the mother. We may be able to save the line by performing in vitro fertilization, intracytoplasmic sperm injection or round spermatid injection followed by embryo transfer to surrogates of a calm strain. How do I prepare/submit DNA/ES cells for microinjection? Customized and detailed protocol will be provided to the user after a consultation. My genetics score was "B+" but I'm having a difficult time calculating the number of animals needed to produce double knockout mice in my animal protocol. Please stop by our office any time. Helpful Links Please refer to the following websites to check the availability of already made mutant mice/rats, ES clones, DNA constructs/BAC clones or CRISPR technologies. The Jackson Laboratory Mouse Genome Informatics JAX Mice Database Search Mutant Mouse Regional Resource Centers (MMRRC) International Mouse Phenotyping Consortium (IMPC) INFRAFRONTIER (Mouse Disease Models) University of California, Davis, Knockout Mouse Project, KOMP Repository National BioResource Project Japan (NBRP) Children's Hospital Oakland Research Institute (CHORI) BACPAC Resources Center Tefor Infrastructure (For designing gRNA) sgRNA Scorer (As of 2/1/2017, it’s now version 2.0) Cas9/gRNA Off-Target Searching Tool CHOPCHOP-Selection of CRISPR/Cas9 and other target sites Please consult the Rodent Genetics Core for additional information on other helpful links. Contact the Rodent Genetics Core 8700 Beverly Blvd. Davis Building, Room 5017 Los Angeles, CA 90048 Makoto Katsumata, PhD, Director 310-248-6523 Send a Message Please ensure Javascript is enabled for purposes of website accessibility